No optimization required easy to switch from other entry-level ECL substrates. Part of The Investigator's Annexe program. A cost effective non-commercial ECL-solution for Western blot detections yielding strong signals and low background We compared several alternative ECL solutions for Western blot detection of endogenous proteins in whole cell lysates using inexpensive, commercially available reagents. The ECL Western Blotting Substrate detects and visualizes the presence of picogram (pg) amounts of antigen through the use of photographic or other suitable chemiluminescent imaging methods. The superoxides then convert the luminol to aminothalate ions, which gives rise to the chemiluminescence light at 430nm for visual detection of your protein.įrom the laboratory of Michael A. The chemiluminescent (CL) reaction of luminol (the active ingredient in the buffer) is catalyzed by superoxide anions which are produced emzymatically from H 20 2. The most popular method used to visualize a protein at the nanogram level involves enhanced chemiluminescence or ECL. Thermo Scientific SuperSignal West Femto Maximum Sensitivity Substrate is an ultra-sensitive enhanced chemiluminescent (ECL) substrate for low femtogram protein level detection by western blot analysis with horseradish peroxidase (HRP) enzyme. We have also found several other blocking agents which work well for. The technique uses three elements: (1) separation by size, (2) transfer to a solid support, and (3) marking the target protein with specific primary and secondary antibody to visualize. We recommend the ECL Blocking Agent at 5 dilution in PBST or TBST (0.1 Tween 20) buffer. Western blot is a widely used analytical technique to detect specific proteins from a complex mixture of proteins extracted from cells or tissue. Western blot detection kit Chemiluminescent substrate based on luminol High sensitivity Detection of antigens with very low concentrations Long signal. Increased signal stability over time (~3hrs).Faster exposure times (10-20 sec, compared to 2+ min).Increased sensitivity and signal, compared to other commercial ECL reagents.Buffered ECL reagent with optimized pH, provides a stronger and more stable chemiluminescent signal for western blots, compared to other commercial ECL reagents.
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