No optimization required easy to switch from other entry-level ECL substrates. Part of The Investigator's Annexe program. A cost effective non-commercial ECL-solution for Western blot detections yielding strong signals and low background We compared several alternative ECL solutions for Western blot detection of endogenous proteins in whole cell lysates using inexpensive, commercially available reagents. The ECL Western Blotting Substrate detects and visualizes the presence of picogram (pg) amounts of antigen through the use of photographic or other suitable chemiluminescent imaging methods. The superoxides then convert the luminol to aminothalate ions, which gives rise to the chemiluminescence light at 430nm for visual detection of your protein.įrom the laboratory of Michael A. The chemiluminescent (CL) reaction of luminol (the active ingredient in the buffer) is catalyzed by superoxide anions which are produced emzymatically from H 20 2. The most popular method used to visualize a protein at the nanogram level involves enhanced chemiluminescence or ECL. Thermo Scientific SuperSignal West Femto Maximum Sensitivity Substrate is an ultra-sensitive enhanced chemiluminescent (ECL) substrate for low femtogram protein level detection by western blot analysis with horseradish peroxidase (HRP) enzyme. We have also found several other blocking agents which work well for. The technique uses three elements: (1) separation by size, (2) transfer to a solid support, and (3) marking the target protein with specific primary and secondary antibody to visualize. We recommend the ECL Blocking Agent at 5 dilution in PBST or TBST (0.1 Tween 20) buffer. Western blot is a widely used analytical technique to detect specific proteins from a complex mixture of proteins extracted from cells or tissue. Western blot detection kit Chemiluminescent substrate based on luminol High sensitivity Detection of antigens with very low concentrations Long signal. Increased signal stability over time (~3hrs).Faster exposure times (10-20 sec, compared to 2+ min).Increased sensitivity and signal, compared to other commercial ECL reagents.Buffered ECL reagent with optimized pH, provides a stronger and more stable chemiluminescent signal for western blots, compared to other commercial ECL reagents.
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Ones that you’d never expect to be on a bee! ![]() As long as you keep it clean and friendly! Bee puns are a very humorous name style for bees, but so are ordinary simple names. There is something really great about picking a funny name. A fun way of picking a name for a striped bee is to use the name of another creature with stripes, or even an object or landmark! And what better way to celebrate your brilliant bee than by honoring those lovely lines. The bold stripes that range between types and individuals from a bright yellow to a deep orange are incredible. One of the most distinctive things about bees is their pattern. Ones that bee-fit their royal status! Whether you want to use a regal title or the name of a past or present queen, here are some lovely ideas for the most important lady in your hive.Ī fun way to get creative with names for your swarm is to use the power of alliteration! Names that also start with B (or H for your honey bee) are a lovely way to find some less common bee names. I think Queen bees deserve seriously majestic names. These names for bumble bees all capture a part of the magic of the biggest bee in your flower garden. They are easy to see floating around the backyard, and cuddly bumble bees make some of the cutest toys ever. Slow and graceful movements make even those with a bit of an insect phobia fairly relaxed in their company. Their large size and soft features make them incredibly endearing. And even their amazing work ethic!īeautiful bumble bees are the embodiment of beauty. Honey bee names can be inspired by their main occupation, or by their gorgeous golden coloration. ![]() Taking a normal name and adding a bit of ‘bee’ magic is a great way to turn a nice name into something really adorable for your little bee.Īnother lovely way to think up cute names for your bee is to look at the sweet world of candies! Watch cute bees acting all friendly! Cute Names for Bees So they have to run or they are both doomed. But, the d/dy treats them equally like dirt beneath its feet, and can take them both out with a single blow. So, in the meme, e x is initially confident because it knows that d/dx can't change it, and it's strong enough to carry the constant along with it and keep it from being sent to 0. The derivative of a constant is 0, so taking d/dy of ce x kills it completely and gives us 0. There is no y on the right hand side of this expression so, as far as y is concerned (in other words, with respect to y), it is a constant function. However, if you take the derivative with respect to y (using d/dy as the differentiation symbol) things are different. In other words, this function is its own slope at every point, which is pretty cool. When you take the derivative of f(x)=ce x (with c being a constant - just a number) with respect to x (so using d/dx as the differentiation symbol), it doesn't change you get dy/dx=f'(x)=ce x. Upload new template Popular My Thanos Simple Calculus Blank View All Meme Templates (1,000s more. Hopefully that answers at least some of your questions! Thanos Simple Calculus Meme Generator The Fastest Meme Generator on the Planet. I'd recommend looking this one up as it gets a little complicated, but the gist of it is that even though dy/dx is a symbol, not a fraction, you can separate the dy and dx to two different functions to represent how the x value changes with one function and how the y value changes with another (keep in mind, y is dependant on x, so there must be some relationship where you can just write the changes independently rather than as a ratio like the normal derivative). The second part of your question is about separation of variables. And that's what the derivative is - how does y change with respect to x at any point (i.e. It should be noted that this isn't just a simple fraction - the "dy/dx" dont refer to specific values of x and y, but rather "an infinitesimally small step of y at a point" and "an infinitesimally small step of x at a point". Essentially, dy/dx simply means "how is y changing with respect to how x is changing over infinitesimally small values across the function?". He believed notations was incredibly important in helping mathematicians understand and create new theorems. With respect to given 'potentials' (a 'vector potential' $A$ and scalar potential $\varphi$), the electric field, or basically, the value at each point in space of the force of the particle divided by charge, or, sticking a charge in space, allows one via the equation $F=qE$ (q is the charge of the particle) to calculate the force on the particle.D/dx is Leibniz notation. This gives rise to the energy released via fusion. This is basically the $E=mc^2$ which describes the energy of the system being in a manner 'equivalent to energy'. ![]() This formula describes the total energy of a system with rest mass, or the mass of the object at rest, $m$, and momentum $p$, where $c$ is the speed of light. This describes, with respect to another inertial frame traveling at a velocity $v$ ($\beta=v/c$), where $c$ is the transmission velocity of light in a local inertial frame, in a vacuum, the time relative to you of the observer moving. ![]() This is the Pythagorean theorem, and corresponds to the right triangle labeled above. However, here is a partial transcription, equation by equation: I don't think anyone has tried to identify these equations from the film, so I'm pleased that the OP here actually cares what was written over the Byonce photograph! I spent nearly 20 hours covering every square inch of the bathroom shower walls with genuine equations and graphs actually related to the solid-state physics discussed by the main character. I was a consultant on the movie Raising Genius about a high-school science genius who locks himself in a bathroom and covers the walls with equations. Thank you Dont forget to confirm subscription in your email. Relation between electric field and electric potentials in electrodynamicsĪs a personal aside, I was responsible for something closely related to this list.Coordinate transformation in special relativity.Thank you brave cops, this world would be terrible without you. Legendre transformation between Lagrangian and Hamiltonian in theoretical mechanics 133 Posts - See Instagram photos and videos from Calculus Memes (calculusmemes).Time evolution of a Heisenberg operator in quantum mechanics.Raising and lowering operators for quantum angular momentum. ![]() ![]() Differential cross section to the scattering amplitude in scattering theory (thanks to Raising operator for the quantum harmonic oscillator.Area under a Gaussian (Normal distribution). ![]() The idea of using so many digits until the result stabilizes seem reasonable. Calculus is concerned with two basic operations, differentiation and integration, and is a tool used by engineers to determine such quantities as rates of change and areas in fact, calculus is the mathematical ‘backbone’ for dealing with problems where variables change with time or some other reference variable and a basic understanding of calculus is essential for further study. For example, it can be used to calculate the velocity and acceleration of a. Applications of Calculus to Biology and Medicine: Case Studies from Lake Victoria is designed to address this issue: it prepares students to. The derivative has many applications in physics, engineering, and economics. It's also useful for determining various infinite sums. Biology majors and pre-health students at many colleges and universities are required to take a semester of calculus but rarely do such students see authentic applications of its techniques and concepts. Also learn how to apply derivatives to approximate function values and find limits using L’Hpital’s rule. ![]() This becomes very useful when solving various problems that are related to rates of change in applied, real-world, situations. Applications of Differential Calculus.notebook 1 Applications of Differential Calculus.notebook 2 Ex 1: Applications of Differential Calculus.notebook 3 Applications of Differential Calculus.notebook 4 Ex 2: Ex 3: Applications of Differential Calculus.notebook 5 Ex 4: Ex 5: Homework: p421 Ex 17A. From the making of a water pump to a Ferris wheel, vector Calculus is required. But by representing $y$ as a Taylor series $\sum a_nx^n$, we can shuffle things around and determine the coefficients of this Taylor series, allowing us to approximate the solution around a desired point. Derivatives describe the rate of change of quantities. find the total flux of electromagnetic fields. To solve this for $y$ would be difficult, if at all possible. Fractional calculus is now a well-established tool in engineering science, with very promising applications in materials modelling. ![]() One reason is that we can approximate solutions to differential equations this way: For example, if we have Typically, Epic gives away one or two free games each week, so now's your chance to stock up and play some new games over the holidays. From now through December 29, you can claim one free game every day on the Epic Games Store. but it would be great to see at list some of these games on the Epic Store Giveaway list.The Epic Games Store is once again hosting its 15 Days of Christmas giveaway event. First of all, yes it is not proven or confirmed by any means. This is the main speculation on all gaming social platforms.
A plain gray footrest or a black pouffe will blend in with most interior styles and give you no trouble matching your sofa or armchair. We also offer plain ottomans for a more understated look. ![]() ![]() Or a blue velvet satin footstool can add that cool hotel-lounge look to your living room. For instance, a rattan pouffe can provide great rustic contrast to an otherwise fabric-heavy design. In our range, you’ll find ottomans in bold colors, distinct textures, and fun shapes that command your attention. Ottomans in many stylesĪside from being practical and versatile, an ottoman or pouffe can be great pieces of decorative furniture. A sturdy ottoman can also double as a comfortable footstool (which can help maintain the family peace on sofa chaise lounge seating disagreements).Ĭheck out our wide range of ottomans, pouffes, and footstools to find the one that’s right for your home. Put down a tray on top and it becomes a snack table for family movie night. Bring it out when entertaining guests to provide an extra seat. It offers seating, storage and style, all in one elegant package. So I caved.Every home can make great use of an ottoman or pouffe. This plus time is the formula for KNEE PAIN! Who knew your knees and legs could hurt so badly without spending a minute at the gym? The VIVO Ergonomic Height Adjustable Foot rest Relief Platform (aka foot rest) has saved my life at the office by relieving this excruciating knee pain! At first glance, you look at this thing, and it's like, $40 for two pieces of metal and a furry surface? No way! But the pain was so excruciating, and other foot rests are seemingly small with no adjustability. This means a greater portion of your legs' weight is suspended by the knees, and less is resting on the floor. Promising review: "Have you ever had to sit in an office chair for about 5 million hours for work? Worst of all, with high desks, you will willingly or unwillingly end up elevating your office chair. I highly recommend this to any size/height person looking to give their legs and feet a varied break throughout the workday. This truly is a special product, built with innovation and practicality in mind. I even use this foot stool as an Achilles/fascia stretch aide by placing it in front of a wall, standing on it facing the wall, and then rolling my toes upward/heels downward. It's also great to be able to flex the feet in BOTH directions throughout the day (toes down or toes up). The height is perfect, and you can even slide your feet more or less centered in order to change the height of your heels from the floor. This wooden stool has a unique design that allows you to roll your ankle to any position you want throughout the day. I use this footstool at work (after having tried all of the plastic/adjustable ones that were stiff, limiting, and broke in less than a year each time). My 220-pound husband stood on it for a few minutes, and the stool held his weight steadily. Promising review: "This foot rest is absolutely well built and practical. StrongTek is a small business started by two brothers, a former data scientist and NASA engineer, to help solve their chronic back pain. I think the price is right for what you're getting." - kellyg This is not a space heater so you're not going to get the same level of heat, but a pair of socks will make a world of difference! I am in my office about 60+ hours a week so this has been a great addition to my desk to make me more comfortable. ![]() I would purchase another one in a heartbeat. In the summer I use the cooling function and it works well. I have had my foot rest for a year now and it's still going strong. The legs are adjustable and you can set it at a height that works for you. I am about 5'2" and have had no problem getting this to a comfortable height that I can work from and not have any issues. ![]() Most of the time I keep it on high and I am fine for a 8–10 hour shift that I work at my desk. Not only does this foot rest keep me comfortable at my desk, but it warms me up. Promising review: "I work in a cold office all day long and on those freezing winter days I am glad I have this. For more products to warm up your perpetually cold hands and feet, we've got this list. This study highlights the Chinese learning needs of L2 preschoolers and provides understanding of their abilities in mapping among character written forms, sounds, and meanings. In addition, results indicate that diverging gaps between L1 and L2 learners’ Chinese character acquisition existed across class levels for the associations involving written character form, but not in regard to associations between character meaning and sound. Results showed that L2 learners had greater development in meaning and sound associations across class levels, implying that they may first develop oral language related abilities. The CCAA is comprised of six subtests and assesses children’s abilities to make associations among character written form (orthography), sound, and meaning. This study used the Chinese Character Acquisition Assessment (CCAA) to compare character acquisition of 491 L2 children aged from 3 to 6 years against that of 240 of their L1 peers from Hong Kong kindergartens. The findings raise the question of whether L2 learners who fall behind their L1 peers in language abilities will be further disadvantaged, showing a weaker development gradient. ![]() ![]() Studies that compared the language abilities of first language (L1) and L2 students in English-speaking contexts have reported young L2 learners’ disadvantage in using the dominant language in oral language and comprehension at school entry. These children face challenges in acquiring L2, especially in Hong Kong, where the dominant Chinese language contrasts strongly with their home languages. 2Department of Pedagogical, Curricular and Professional Studies, University of Gothenburg, Gothenburg, Swedenįor culturally and linguistically diverse children, early second language (L2) development is important for school achievement and social inclusion.1Faculty of Education, The University of Hong Kong, Pokfulam, Hong Kong SAR, China.Chan 1*, Wai Ming Cheung 1 and Ference Marton 2 Sign up today and make full use of the most effective activities designed to teach your child to read through phonics.Stephanie W. It is an absolute joy to watch your child begin the baby steps of combining letters and sounds to read unfamiliar words. Parents, both native and non-native English speakers, can follow our guidelines and phonics activities to bring kids from zero to hero in reading. Teaching kids to read is not solely the job of the classroom teacher. As learners are different, you need a varied set of resources to teach phonics – phonics worksheets, phonics games, phonics flashcards, listening materials, word families activities, phonics short stories, phonics lesson videos, and PowerPoint – we got you covered! It is a more effective method for learning to read compared to word recognition. Phonics teaches kids to read unfamiliar words. ![]() Reading comprehension is not an immediate priority at the early phases. In a synthetic phonics approach, kids blend sounds to read full words. These letters can be combined to form sounds called phonemes. As such, preschoolers usually begin with print awareness using the 26 letters of the alphabet. ![]() Phonics is a method of teaching children to read by combining letters to form sounds. Deliver the joy of learning to read by using the Kiz Phonics curriculum and phonics activities. Watch Me Make This Cheesy Tater Tot Casserole: When you are ready to serve it, simply remove the casserole from the freezer and allow it to sit at room temp for half an hour, then bake as directed. Finally, place the entire dish in an X-large freezer bag (if you have one). Next, lay cling wrap on the top of the ingredients, then wrap the dish in heavy-duty aluminum foil. I like to use latex gloves to combine the tots and the sauce- it makes this so much easier! After everything is well-mixed, place the mixture in a baking dish (but do not bake). To begin, simply mix the sauce ingredients, fold in the tater tots, and combine well. This cheesy tater tot casserole can be made ahead as long as you do not let the tater tots thaw. Can A Cheesy Tater Tot Casserole Be Made Ahead? Leftover tots can be put in the freezer for later use. ![]() Whichever brand you choose, you’ll use a whole bag and part of another to get two pounds. Alexia’s potato puffs are more sophisticated and flavorful than plain tater tots and have a fantastic flavor and a light, crisp texture. While I love Ore Ida, my personal favorite version is the Alexia brand of potato puffs. Of all their tater tots, my favorites are the tiny tots and the extra crunchy tots. Ore Ida has the trademark on tater tots, and these tots will work great in this recipe. As a result, this tasty recipe boasts a more flavorful, sophisticated flavor. While many tater tot casserole recipes call for cream of mushroom soup, the base of this cheesy sauce may surprise you! Canned cheddar cheese soup and French onion dip are the secret ingredients in this dish! By mixing these two ingredients, the result is a cheese-fondue-like sauce. What Is The “Secret Sauce” In Cheesy Tater Tot Casserole? My version is cheesy, savory, and crowd-pleasing every time! The end result was SO delicious! I think it tastes more sophisticated than traditional tater tot casseroles that contain mushroom or chicken soup. This crazy yummy, cheesy dish was born when I baked my favorite potato puffs in a sauce similar to my cheese fondue. ![]() One of my favorite appetizers, French Onion Cheese Fondue, inspired this tater tot casserole recipe. This hearty, savory dish is always a crowd-pleaser!Įasy, Cheesy Tater Tot Casserole Is My Personal Creation. You will LOVE this recipe for the easiest and cheesiest tater tot casserole (with no cream of mushroom soup). For example, the regexp pattern 1 matches the string “1”, and the pattern boy matches the string “boy”. In a regular expression, most characters - including letters and numbers - represent themselves. Technically speaking, the word or phrase patterns are regular expressions-just very simple ones. A regular expression - or “regexp”- is a text string of special characters that specifies a set of patterns to match. In addition to word and phrase searches, you can use grep to search for complex text patterns called regular expressions. NOTE: These regexps were quoted with characters this is because some shells otherwise treat the “ˆ”character as a special “metacharacter.” To output lines in the file “book” that contain a “$” character, type: The following samples describe some regexp examples for commonly searched-for patterns. Many examples discussed in this article have practical implications meaning you can use them in your daily Linux programming. In this article, we show you to run advance string searching using Grep with regular expression by giving you 10 hands-on examples on its implementations. Eventually, the grep command was written to do this search on a file when not using ed. The name “grep” derives from a command in the now-obsolete Unix ed line editor tool - the ed command for searching globally through a file for a regular expression and then printing those lines was g/re/p, where re was the regular expression you would use. The grep Linux/Unix command line utility is one of most popular tools for searching and finding strings in a text file. ![]() He is also a senior instructor and developer living in Washington DC. He is a senior editor at Touchstone Words where he writes and reviews coding and technology articles. He has written extensively on advanced topics on web design, mobile app development and blockchain. Matt Zand is the founder of High School Technology Services, DC Web Makers and Coding Bootcamps. |
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